Fig. 1
C58/J mice exhibit reduced sociability and downregulation of immune-related pathways in amygdala compared to C57BL/6J mice. (A) Comparison of social preference based on time spent in close proximity to a stranger mouse during the 3-chamber social choice test in male (n = 4 per group) and female (n = 4 per group) C57BL/6J (B6), C58/J high social (58 H), and C58/J low social (58 L) mice. Preference for social interaction was assessed at postnatal weeks 7–8. Results reported as mean ± SEM, *p < 0.05 using Mann-Whitney U test. (B) Volcano plot of differentially expressed genes obtained from bulk RNA-Seq analysis of amygdala from C58/J and C57BL/6J mice (n = 4 mice per group). Genes with an adjusted P-value < 0.05 and Log2 Fold Change of > 2 and < -2 were differentially expressed. 64 genes were upregulated and 63 genes were downregulated in C58/J. (C) GO enrichment analysis of Bulk RNA-Seq data from C58/J and C57BL/6J amygdala. Pathways shown were significant (FDR q-value < 0.05) and were downregulated in C58/J mice. (D) Significant (q-value < 0.05) hypermethylated (Mir692-2, Niban3, Ppp1r2, Lcmt2) and hypomethylated (Cxcl1, Akap9) regions identified in C58/J compared to C57BL/6J mice (n = 4 mice per group). Lines represent individual smoothed methylation level estimates for C58/J (C58J, red) or C57BL/6J (B6, blue). Dots represent methylation level estimates of an individual CpG in a single sample, and dot size is representative of coverage. CpG and genic annotation tracks are shown below each plot