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Fig. 1 | Behavioral and Brain Functions

Fig. 1

From: Cofilin linked to GluN2B subunits of NMDA receptors is required for behavioral sensitization by changing the dendritic spines of neurons in the caudate and putamen after repeated nicotine exposure

Fig. 1

Nicotine decreases pCofilin- and pLIMK1 levels in the CPu. (A) Areas of the CPu punched out for conducting western blot analysis within anterior to posterior (AP) coordinates of + 1.2 to + 0.8 mm. (B, C) Repeated nicotine exposure decreased pCofilin- and pLIMK1-IR at the 1 h time point. (D) A brain section for conducting immunofluorescence staining within the AP coordinates of + 1.2 to + 0.8 mm. The red stars in the CPu indicate the areas captured in photomicrographs shown in Figure E. (E) Repeated nicotine exposure decreased pCofilin-IR in MSN of the CPu. The scale bar represents 30 μm. The arrows in the magnified images indicate puncta. The scale bar represents 10 μm. (F, G) Quantitative analysis showed that repeated nicotine exposure decreased the number of co-localized puncta of pCofilin- and DARPP-32-IR but not the number of pCofilin-positive neurons in the CPu. N = 4 rats per group. *p < 0.05

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Behavioral and Brain Functions

ISSN: 1744-9081

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